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restriction enzymes asci  (New England Biolabs)


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    Structured Review

    New England Biolabs restriction enzymes asci
    Restriction Enzymes Asci, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 1247 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/restriction+enzymes+asci/AscI/pmc12969024-258-13-16
    Average 97 stars, based on 1247 article reviews
    restriction enzymes asci - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Identification of fidelity-determined residues of Porcine reproductive and respiratory syndrome virus through structural alignment
    Article Snippet: .. The mutated fragment was then cloned into the backbone plasmid, digested with restriction enzymes AscI and NheI-HF (NEB, R0558 and R3131), and assembled via homologous recombination using the ClonExpress MultiS one-step cloning kit (Vazyme, C113-02) to generate the full-length infectious clone plasmid. ..

    Article Title: The jojoba lipid droplet protein LDAP1 facilitates the packaging of wax esters into lipid droplets
    Article Snippet: .. Fragments were then fused using an Overlap PCR and cloned into the plant expression vector pMDC32mCherry ( ) using the restriction enzymes AscI and PacI (NEB), and T4 ligase (NEB). .. AtLDAP1 and ScLDAP1 mutants were synthesized by the DNA synthesis services provided by Gene Universal Inc.

    Article Title: The jojoba lipid droplet protein LDAP1 facilitates the packaging of wax esters into lipid droplets
    Article Snippet: AtLDAP1 and ScLDAP1 mutants were synthesized by the DNA synthesis services provided by Gene Universal Inc. .. Genes were amplified using the appropriate forward and reverse primers from vectors containing synthesized genes, and cloned into pMDC32mCherry ( ) using the restriction enzymes AscI and PacI (NEB) and T4 ligase (NEB). .. The binary vectors for simultaneous expression of genes encoding MaFAR, ScWS, and ScLDAP1 were generated using the Gateway technology (Thermo Fisher Scientific) as previously described ( ).

    Plasmid Preparation:

    Article Title: Identification of fidelity-determined residues of Porcine reproductive and respiratory syndrome virus through structural alignment
    Article Snippet: .. The mutated fragment was then cloned into the backbone plasmid, digested with restriction enzymes AscI and NheI-HF (NEB, R0558 and R3131), and assembled via homologous recombination using the ClonExpress MultiS one-step cloning kit (Vazyme, C113-02) to generate the full-length infectious clone plasmid. ..

    Article Title: The jojoba lipid droplet protein LDAP1 facilitates the packaging of wax esters into lipid droplets
    Article Snippet: .. Fragments were then fused using an Overlap PCR and cloned into the plant expression vector pMDC32mCherry ( ) using the restriction enzymes AscI and PacI (NEB), and T4 ligase (NEB). .. AtLDAP1 and ScLDAP1 mutants were synthesized by the DNA synthesis services provided by Gene Universal Inc.

    Article Title: Host cells and their use for producing ribitol and further monosaccharides
    Article Snippet: .. The resulting fragment was subcloned into the same vector used for cloning of expressing of wild type ribitol dehydrogenases by restriction digestion with restriction enzymes AscI and SphI (New England Biolabs, Ipswich, Massachusetts), followed by ligation and verification as described above. ..

    Homologous Recombination:

    Article Title: Identification of fidelity-determined residues of Porcine reproductive and respiratory syndrome virus through structural alignment
    Article Snippet: .. The mutated fragment was then cloned into the backbone plasmid, digested with restriction enzymes AscI and NheI-HF (NEB, R0558 and R3131), and assembled via homologous recombination using the ClonExpress MultiS one-step cloning kit (Vazyme, C113-02) to generate the full-length infectious clone plasmid. ..

    Cloning:

    Article Title: Identification of fidelity-determined residues of Porcine reproductive and respiratory syndrome virus through structural alignment
    Article Snippet: .. The mutated fragment was then cloned into the backbone plasmid, digested with restriction enzymes AscI and NheI-HF (NEB, R0558 and R3131), and assembled via homologous recombination using the ClonExpress MultiS one-step cloning kit (Vazyme, C113-02) to generate the full-length infectious clone plasmid. ..

    Article Title: Host cells and their use for producing ribitol and further monosaccharides
    Article Snippet: .. The resulting fragment was subcloned into the same vector used for cloning of expressing of wild type ribitol dehydrogenases by restriction digestion with restriction enzymes AscI and SphI (New England Biolabs, Ipswich, Massachusetts), followed by ligation and verification as described above. ..

    Polymerase Chain Reaction:

    Article Title: The jojoba lipid droplet protein LDAP1 facilitates the packaging of wax esters into lipid droplets
    Article Snippet: .. Fragments were then fused using an Overlap PCR and cloned into the plant expression vector pMDC32mCherry ( ) using the restriction enzymes AscI and PacI (NEB), and T4 ligase (NEB). .. AtLDAP1 and ScLDAP1 mutants were synthesized by the DNA synthesis services provided by Gene Universal Inc.

    Expressing:

    Article Title: The jojoba lipid droplet protein LDAP1 facilitates the packaging of wax esters into lipid droplets
    Article Snippet: .. Fragments were then fused using an Overlap PCR and cloned into the plant expression vector pMDC32mCherry ( ) using the restriction enzymes AscI and PacI (NEB), and T4 ligase (NEB). .. AtLDAP1 and ScLDAP1 mutants were synthesized by the DNA synthesis services provided by Gene Universal Inc.

    Article Title: The jojoba lipid droplet protein LDAP1 facilitates the packaging of wax esters into lipid droplets
    Article Snippet: .. Restriction enzymes AscI and PacI (NEB) and T4 ligase (NEB) were used to insert amplified DNA into the plant expression vectors pMDC32 and pMDC32mCherry ( ). ..

    Article Title: Host cells and their use for producing ribitol and further monosaccharides
    Article Snippet: .. The resulting fragment was subcloned into the same vector used for cloning of expressing of wild type ribitol dehydrogenases by restriction digestion with restriction enzymes AscI and SphI (New England Biolabs, Ipswich, Massachusetts), followed by ligation and verification as described above. ..

    Amplification:

    Article Title: The jojoba lipid droplet protein LDAP1 facilitates the packaging of wax esters into lipid droplets
    Article Snippet: .. Restriction enzymes AscI and PacI (NEB) and T4 ligase (NEB) were used to insert amplified DNA into the plant expression vectors pMDC32 and pMDC32mCherry ( ). ..

    Article Title: The jojoba lipid droplet protein LDAP1 facilitates the packaging of wax esters into lipid droplets
    Article Snippet: AtLDAP1 and ScLDAP1 mutants were synthesized by the DNA synthesis services provided by Gene Universal Inc. .. Genes were amplified using the appropriate forward and reverse primers from vectors containing synthesized genes, and cloned into pMDC32mCherry ( ) using the restriction enzymes AscI and PacI (NEB) and T4 ligase (NEB). .. The binary vectors for simultaneous expression of genes encoding MaFAR, ScWS, and ScLDAP1 were generated using the Gateway technology (Thermo Fisher Scientific) as previously described ( ).

    Electrophoresis:

    Article Title: Trends and prevalence of Listeria monocytogenes at poultry abattoirs in Taiwan 2014-2024.
    Article Snippet: Listeria monocytogenes (L. monocytogenes) is a Gram-positive bacterium and a significant foodborne pathogen that causes listeriosis and occurs in animal products and other foods.. Information on the prevalence of L. monocytogenes in poultry carcasses at abattoirs across Taiwan is limited.. This study investigated L. monocytogenes contamination in poultry carcasses at abattoirs across Taiwan from 2014 to 2024, covering chickens (2014–2024), ducks (2019–2024) and geese (2020–2024) slaughter lines.

    Ligation:

    Article Title: Host cells and their use for producing ribitol and further monosaccharides
    Article Snippet: .. The resulting fragment was subcloned into the same vector used for cloning of expressing of wild type ribitol dehydrogenases by restriction digestion with restriction enzymes AscI and SphI (New England Biolabs, Ipswich, Massachusetts), followed by ligation and verification as described above. ..

    Synthesized:

    Article Title: The jojoba lipid droplet protein LDAP1 facilitates the packaging of wax esters into lipid droplets
    Article Snippet: AtLDAP1 and ScLDAP1 mutants were synthesized by the DNA synthesis services provided by Gene Universal Inc. .. Genes were amplified using the appropriate forward and reverse primers from vectors containing synthesized genes, and cloned into pMDC32mCherry ( ) using the restriction enzymes AscI and PacI (NEB) and T4 ligase (NEB). .. The binary vectors for simultaneous expression of genes encoding MaFAR, ScWS, and ScLDAP1 were generated using the Gateway technology (Thermo Fisher Scientific) as previously described ( ).

    Control:

    Article Title: Intravenous oncolytic vaccinia expressing transgenes for enhanced safety, inhibition of shedding, imaging, and systemic cancer immunotherapy
    Article Snippet: Codon-optimized transgene sequences of WT HSV-TK and HSV-TK variants TK.007, SR39, and dm30 were synthetized with the P-SE/L synthetic early-late promoter and homologous flanking regions for the ΔJ2R insertion site (GenScript, Piscataway, NJ) and liberated from the pUC57 plasmid via PCR amplification. .. Viral genomic DNA from the control virus was purified and digested with the restriction enzymes AscI (NEB, Ipswich, MA, R0558) and AsiSI (NEB R0630), targeting cut sites engineered into regions flanking the ΔJ2R insertion site. .. To construct the HSV-TK variants, viral genomic DNA from TK.WT containing WT HSV-TK was used as a template and cut using CRISPR-Cas guide RNAs (IDT) targeting DNA sequences in the WT HSV-TK gene.

    Article Title: Intravenous Oncolytic Vaccinia Expressing Transgenes for Enhanced Safety, Inhibition of Shedding and Systemic Cancer Immunotherapy
    Article Snippet: 393 All the modifications in the viruses described in Table 1 were generated using the same 394 procedure by recombination and reactivation of vaccinia virus DNA.39 Codon optimized 395 transgene sequences of wild type (WT) HSV-TK and HSV-TK variants TK.007, SR39, and 396 dm30 were synthetized with the P-SE/L synthetic early-late promoter and homologous flanking 397 regions for the ΔJ2R insertion site (GenScript, Piscataway, NJ) and liberated from the pUC57 398 plasmid via PCR amplification. .. Viral genomic DNA from the control virus was purified40 and 399 digested with restriction enzymes AscI (NEB, Ipswich, MA, R0558) and AsiSI (NEB R0630) 400 targeting cut sites engineered into regions flanking the ΔJ2R insertion site. .. To construct the 401 HSV-TK variants, viral genomic DNA from TK.WT containing WT HSV-TK was used as 402 template and cut using CRISPR/Cas guide RNAs (IDT) targeting DNA sequences in the WT 403 HSV-TK gene.

    Virus:

    Article Title: Intravenous oncolytic vaccinia expressing transgenes for enhanced safety, inhibition of shedding, imaging, and systemic cancer immunotherapy
    Article Snippet: Codon-optimized transgene sequences of WT HSV-TK and HSV-TK variants TK.007, SR39, and dm30 were synthetized with the P-SE/L synthetic early-late promoter and homologous flanking regions for the ΔJ2R insertion site (GenScript, Piscataway, NJ) and liberated from the pUC57 plasmid via PCR amplification. .. Viral genomic DNA from the control virus was purified and digested with the restriction enzymes AscI (NEB, Ipswich, MA, R0558) and AsiSI (NEB R0630), targeting cut sites engineered into regions flanking the ΔJ2R insertion site. .. To construct the HSV-TK variants, viral genomic DNA from TK.WT containing WT HSV-TK was used as a template and cut using CRISPR-Cas guide RNAs (IDT) targeting DNA sequences in the WT HSV-TK gene.

    Article Title: Intravenous Oncolytic Vaccinia Expressing Transgenes for Enhanced Safety, Inhibition of Shedding and Systemic Cancer Immunotherapy
    Article Snippet: 393 All the modifications in the viruses described in Table 1 were generated using the same 394 procedure by recombination and reactivation of vaccinia virus DNA.39 Codon optimized 395 transgene sequences of wild type (WT) HSV-TK and HSV-TK variants TK.007, SR39, and 396 dm30 were synthetized with the P-SE/L synthetic early-late promoter and homologous flanking 397 regions for the ΔJ2R insertion site (GenScript, Piscataway, NJ) and liberated from the pUC57 398 plasmid via PCR amplification. .. Viral genomic DNA from the control virus was purified40 and 399 digested with restriction enzymes AscI (NEB, Ipswich, MA, R0558) and AsiSI (NEB R0630) 400 targeting cut sites engineered into regions flanking the ΔJ2R insertion site. .. To construct the 401 HSV-TK variants, viral genomic DNA from TK.WT containing WT HSV-TK was used as 402 template and cut using CRISPR/Cas guide RNAs (IDT) targeting DNA sequences in the WT 403 HSV-TK gene.

    Purification:

    Article Title: Intravenous oncolytic vaccinia expressing transgenes for enhanced safety, inhibition of shedding, imaging, and systemic cancer immunotherapy
    Article Snippet: Codon-optimized transgene sequences of WT HSV-TK and HSV-TK variants TK.007, SR39, and dm30 were synthetized with the P-SE/L synthetic early-late promoter and homologous flanking regions for the ΔJ2R insertion site (GenScript, Piscataway, NJ) and liberated from the pUC57 plasmid via PCR amplification. .. Viral genomic DNA from the control virus was purified and digested with the restriction enzymes AscI (NEB, Ipswich, MA, R0558) and AsiSI (NEB R0630), targeting cut sites engineered into regions flanking the ΔJ2R insertion site. .. To construct the HSV-TK variants, viral genomic DNA from TK.WT containing WT HSV-TK was used as a template and cut using CRISPR-Cas guide RNAs (IDT) targeting DNA sequences in the WT HSV-TK gene.



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